Histology Society of Ohio Symposium/Convention
April 22-23 2022

Embassy Suites by Hilton
Columbus, OH 43231

Lecture 1
(12 - 1:00 pm)

Biological Effects of Electronic Cigarettes: Are they Bad for Your Lungs?

Min-Ae Song, Ph.D., Assistant Professor of Practice, Division of Environmental Health Sciences, College of Public Health

The use of electronic cigarettes (E-cigs) has skyrocketed in both adolescents and young adults, among both current- and former-smokers as smoking cessation devices. However, for now, the effects of acute and chronic exposure to e-cigs are poorly understood, particularly in the target organ. In the presentation, I will discuss my current research on the biological effects of e-cig use in the human lungs to provide insights into shared and unique toxic effects of e-cigs compared to cigarette smoking.

Lecture 2
(12 - 1:00 pm)

Interpretation of Commonly Used Special Stains

Linda McDonald, HT (ASCP) Cleveland Clinic Foundation

A basic overview of routine clinical histochemical stains utilized in most Histology Laboratories. We will identify basic staining mechanisms and limited troubleshooting for the stains in discussion. Some clinical applications will also be discussed. The stains covered will be routine connective Tissue, Microorganism, Carbohydrate and Pigment stains.

Lecture 3
(1:15 - 2:15 pm)

Pathology Reporting, Recut: Melting it Down and Embed- ding some Examples

Alexander D. Fitzthum, M.D., Assistant Professor - Clinical, The Ohio State Wexner Medical Center

Highlighting the terms used by pathologists in their reports, with an em- phasis on morphologic term, synoptic reporting and adequacy. Examples with histologic images will be used throughout.

Lecture 4
(1:15 - 2:15 pm)

Pitfalls and Problems with Head and Neck Specimens

Abberly Lott Limbach, M.D., Assistant Professor, Director, Head &Neck Division, Wexner Medical Center Department of Pathology

Specimens from the head and neck have a unique anatomy and can present unique challenges to the histology lab. In this talk we will review and discuss common specimens in from the head and neck as well as special cases which can present a challenge to the histology lab such as fungal sinusitis, bone, lymph nodes, and thyroid.

Vendor Break 2:15– 3 pm

Lecture 5
(3:00 - 4:00 pm)

Digital Pathology and Artificial Intelligence: Gearing up for a Digital Workflow” A Virtual Live Event

Zaibo Li, M.D., Ph.D., Associate Professor, Associate Director of Pathology Informatics, Department of Pathology, Ohio State University

Objectives of the session:

  • Provide a pathologist perspective of the current state of Whole Slide Imaging for clinical diagnostics including primary diagnosis
  • Provide an understanding of the current state and future directions of digital pathology at The Ohio State University: Lessons learnt and next steps
  • Explore the future directions of pathology’s role in advanced diagnostics including use of image analysis and artificial intelligence

Lecture 6
(3:00 - 4:00 pm)

GBM: An Overview of the Most Common Primary Brain Neoplasm

Peter Kobalka, M.D. Assistant Professor Divisions of Neuropathology, Cytopathology, and Intraoperative Pathology, Associate Director of Surgical Pathology Fellowship, The Ohio State University Medical Center

Glioblastoma, the most common malignant brain tumor in adults, is also one of the most deadly. Most patients die within 2 years of diagnosis and only rarely survive to 5 years. These tumors derive from astrocytes, a common support cell in the CNS, responsible for such functions as maintaining the blood brain barrier. This talk will provide an overview of the cells responsible, etiology and genetic factors, histological subtypes, and an overview of prognosis and current treatment regimens

Vendor Reception 4:00 – 5:30 pm
(Cash Bar, Hot and Cold hors d’oeuvres)


Wound Care in the 21st Century

Dr. Kathleen M. Graytock, DPM, CWS,FCCWS, FAPWC, AAWC, Berry Leaf Foot and Ankle Center, Ltd., Columbus and Mansfield, OH

There are over 3,000 wound care products on the market today. How do we decide what to use and when to use it? It’s not as easy as it sounds.

Lecture 8
(8:00 – 9:00 am)

How to Identify the Basic Tissues with H&E Stain

Amy L. Aulthouse, Ph.D., Professor of Biology, Ohio Northern University

This talk will review the basic tissues and subtypes when viewing slides with routine H&E staining. Special attention will be given to the identifying morphological features that allow for proper identification of the tissues. Lastly, “look a-likes” will be presented with explanation on how to correctly distinguish between different tissues.

Lecture 9
(8:00 – 9:00 am)

Decalcification and the Solutions

Diane L. Sterchi MS, HT/HTL(ASCP)

It is really beneficial for the histotechnologist to know what is in your routine decal solution. Exploring the decal solutions and what chemicals are in them. Acting smart on determining the end point of decalcification easily without destroying the tissue.

Lecture 10
(9:15 - 10:15 am)

Not Just Skin Deep: Histologic Considerations of Cutaneous Specimens

Catherine Chung, M.D., Assistant Clinical Professor of Pathology and Medicine, The Ohio State University Wexner Medical Center and Staff Pathologist at Nationwide Children's Hospital

This talk will address special considerations when preparing skin biopsies and excisions such as inflammatory versus neoplastic lesions, melanocytic versus non-melanocytic tumors, and alopecia protocols. Special sections such as en face sections for "Slow Mohs" procedures in diagnosis of residual melanoma will also be discussed. Lastly, there will be discussion on approaches to the role immunohistochemistry plays in common skin diagnoses.

Lecture 11
(9:15 - 10:15 am)

Fundamentals of Fixation and Tissue Processing

Cindy Sampias, JD CT (ASCP) HTL l Applications Technical Team Lead – Core Histology l Leica Biosystems

This course provides an overview of grossing, fixation and tissue processing, along with a review of the more common artifacts that may be expected when these processes are less than ideal. I also include a short history of tissue processing techniques and tips/trips from my applications field experience

Lecture 12
(11:00 - 12:00 pm)

Neoplasms of Exocrine and Endocrine Pancreas

Wei Chen, M.D., Ph.D., Associate Director, Liver Pathology, GI/Liver Pathology Fellowship, Department of Pathology, The Ohio State University Wexner Medical Center

Pancreatic cancer is the 4th mostly deadly cancer in the USA, with a five year survival rate of 9%. This presentation will review different cancer types from exocrine and endocrine pancreas, focusing on their diverse histologic and immunohistochemical features. New developments in molecular pathology and clinical treatment will also be briefly discussed

Lecture 13
(11:00 - 12:00 pm)

Intraoperative Frozen Section Consultation – Technical As- pects, Clinical Applications and Diagnostic Limitations

Johanna Savage, M.D., Assistant Professor of Pathology, Department of Pathology, The Ohio State University Wexner Medical Center

Frozen section consultations represent a unique aspect of pathology. This presentation will discuss some of the technical and professional aspects of frozen section pathology, its clinical applications and its diagnostic limitations.

Lunch Buffet and Membership Meeting 12:00-1:00pm

Lecture Series 14
(1:15 - 2:15 pm)

Molecular Biomarkers: The Histology Lab is the Tissue Police—They protect and Preserve the DNA and Assure Adequacy of the Sample

Joseph Sreenan, M.D., Director of Graduate Medical Education, Mercy Health St. Rita’s Lima

1) Discuss molecular biomarkers and their use in diagnosis, prognosis and therapeutics in oncology; 2) Define standards for proper fixation of tissue to optimize biomarker recovery; 3) Use a series of cases to discuss ways to ensure adequate tissue to perform biomarker analysis

Every Slide Tells A Story

Sarah Keaton Ph.D. Scientific Liaison, Roche Diagnostics Corporation, Tissue Diagnostics

The process of formalin-fixing and paraffin-embedding tissue specimens in order to assess protein expression by immunohistochemistry is commonly used in laboratories around the world. In this talk, we will discuss the challenges found in the histology process, including tissue collection and handling, fixation, grossing, tissue processing, and embedding. We will discuss the importance of quality within each processing step, the possible sources of variation in the histology process, and where improvements and standardization in processes can be made to improve variability.

Approaches to Immunohistochemistry Protocol Development and Troubleshooting

Elizabeth A. Chlipala, BS, HTL (ASCP) QIHC and Diane Sterchi, MS, HT/HTL (ASCP)

Although immunohistochemistry (IHC) is still a powerful tool for detecting and localizing expression of specific proteins in tissues, protocol development and troubleshooting require an in-depth understanding of the staining process for all species. IHC protocol development for Human and Animal use should be carefully documented and following a process. This helps to establish accurate staining, and standardizes/validates the staining interactions between the antibody, control tissues (positive and negative) and the reagents used to detect the antibody. We will give a general description of thefundamental concepts of IHC staining, discuss a detailed method for developing reliable IHC staining protocols, and present useful approaches to troubleshooting.